E8651
Anti-E2F3 antibody ,Mouse monoclonal
clone PG30, purified from hybridoma cell culture
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PG30, monoclonal
form
buffered aqueous solution
mol wt
antigen 57 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
immunoprecipitation (IP): suitable using denatured E2F3
microarray: suitable
western blot: 4-8 μg/mL using transfected 293T cells expressing E2F3
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... E2F3(1871)
General description
Monoclonal Anti-E2F3 (mouse IgG2a isotype) is derived from the PG30 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human E2F Transcription Factor 3 (E2F3) protein. Transcription factor E2F3 is a DNA-binding protein of ~57 kDa encoded by the E2F3 gene in humans. It belongs to E2F transcription factor family of proteins that bind to the sequence TTTCGCGC.
Immunogen
recombinant human E2F3.
Application
Monoclonal Anti-E2F3 antibody produced in mouse has been used for the following applications:
- immunoprecipitation using denatured E2F3
- western blotting at a concentration of 4-8μg/mL using transfected 293T cells expressing E2F3
- electrophoretic mobility shift assay (EMSA).
Biochem/physiol Actions
Transcription factor E2F3 is involved in the regulation of cell cycle. E2F3 helps in the regulation of the expression of a number of genes that are important in cell proliferation. It is a oncogene with strong proliferative potential and is involved in many processes. E2F3 plays an important role in the development of several types of cancer. Overexpression of E2F3 may be associated with hepatocellular carcinoma (HCC). It plays an important role in controlling cell cycle progression and proliferation. E2F3 is frequently expressed in pediatric Wilms′ tumors and may be associated with the same.
Physical form
Solution in phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Preparation Note
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Cloning and characterization of human DP2, a novel dimerization partner of E2F.
Zhang, et al.
Oncogene, 10(11), 2085-2093 (1995)
Pablo R Olmos et al.
Diabetes research and clinical practice, 71(3), 320-333 (2005-10-20)
After a study of ICA prevalence among relatives of Type-1 diabetics (DM1) in Santiago, Chile, parents of those who tested positive asked us to go on forward with an intervention study. We had screened 1021 relatives, of which 30 had
Xiaoyun Zeng et al.
Oncology reports, 31(3), 1139-1146 (2014-01-10)
E2F transcription factor 3 (E2F3), a member of the E2F transcription factor family and a member of the genes involved in the regulation of cell cycle, is an oncogene with strong proliferative potential. E2F3 is involved in many processes and
Qun An et al.
Journal of pediatric surgery, 48(11), 2187-2193 (2013-11-12)
The transcription factor E2F3 plays an important role in controlling cell cycle progression and proliferation, and is overexpressed in various human cancers. The present study was undertaken to examine the expression of E2F3 and investigate its relevance in clinical and
J A Lees et al.
Molecular and cellular biology, 13(12), 7813-7825 (1993-12-01)
E2F is a transcription factor that helps regulate the expression of a number of genes that are important in cell proliferation. Recently, several laboratories have isolated a cDNA clone that encodes an E2F-like protein, known as E2F-1. Subsequent characterization of
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