D9820

Anti-DOCK1 (C-terminal region) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-DOCK180, Anti-Dedicator of cytokinesis 1, Anti-Downstream of CRK-binding protein, 180-KD
• UNSPSC Code: 12352202
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~180 kDa
• species reactivity: rat, mouse, human
• concentration: ~1.5 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using NIH-3T3 cells; western blot: 2-4 μg/mL using HeLa cell lysate and PC12 cell lysate
• UniProt accession no.: Q14185
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DOCK1(1793)

Description

General description

Dedicator of cytokinesis protein 1 (DOCK1), also termed as DOCK180, is a member of a family of guanine nucleotide exchange factors (GEF). The DOCK1 gene is mapped to human chromosome 10q26.2.

Specificity

Anti-DOCK1 (C-terminal region) specifically recognizes human, rat, and mouse DOCK1.

Application

Anti-DOCK1 (C-terminal region) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Dedicator of cytokinesis protein 1 (DOCK1) acts as an activator of Rac and is involved in cytoskeletal reorganization and cell migration. By activating the β1 and αvβ5 integrins, DOCK1 regulates cell motility, filopodia formation, and phagocytosis. It is involved in the integrin signaling through the adapter molecule crk (Crk)- (p130) Crk-associated substrate p130 Cas complex. Its localization at focal adhesions induces the phosphorylation of p130Cas. DOCK1 forms a complex with engulfment and cell motility protein 1 (ELMO1) that functions as a bipartite guanine nucleotide exchange factor (GEF) for Rac and inhibits ubiquitylation of DOCK1. In mammalian cells, the CrkII-ELMO1-DOCK1 complex has been shown to promote phagocytosis. DOCK1 has been suggested to play a central role in netrin signaling during neuronal development by mediating the attractive responses by neurons to netrin-1. It mediates morphological changes such as integrin-mediated cell spreading and nerve growth factor (NGF)-induced neurite outgrowth. DOCK1 has been found to undergo ubiquitination and proteasome-dependent degradation, a process enhanced in response to epidermal growth factor (EGF), Crk, and adhesion-dependent signals.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.