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D3159

Sigma-Aldrich

Deoxyribonucleic acid from herring sperm

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp), degraded

Synonym(s):

Deoxyribonucleic acid, partially degraded from herring sperm, hsDNA

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About This Item

EC Number:
MDL number:
UNSPSC Code:
41106305
eCl@ss:
32160414
NACRES:
NA.51

Quality Level

form

powder

suitability

not suitable for substrate for typical DNase assays (“crude oligonucleotides", <50 bp)

storage temp.

2-8°C

General description

Herring Sperm DNA, also known as hsDNA is a natural source of DNA designed for DNA binding agent studies. (1) The detection limits of hsDNA are around 5.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-2.5 mµg.

Application

Herring sperm DNA (hsDNA) is a natural DNA used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Herring sperm DNA is also used in physicochemical studies of DNA behavior in solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Po Wang et al.
Biosensors & bioelectronics, 26(7), 3339-3345 (2011-02-08)
A rapid, convenient and accurate method for the simultaneous detection of guanine (G), adenine (A), thymine (T) and cytosine (C) was developed at a multiwalled carbon nanotube (MWCNT)/choline (Ch) monolayer-modified glassy carbon electrode (GCE). X-ray photoelectron spectroscopy data demonstrated that
Yan Zhang et al.
Nucleosides, nucleotides & nucleic acids, 30(1), 49-62 (2011-01-25)
The Mg(II) complex of Schiff base (K[HL]) derived from vanillin and L-tryptophan could bind with herring sperm DNA. The binding behaviors between them in physiological pH environment (pH 7.40) have been studied by spectroscopy, cyclic voltammetry and viscosity methods. Binding
M N Patel et al.
Journal of enzyme inhibition and medicinal chemistry, 26(3), 359-366 (2011-01-29)
A series of ternary copper(II) complexes have been derived using levofloxacin and five phenanthroline derivatives. Complexes were characterized using infrared spectroscopy, Thermogravimetric (TG)-analysis, fast atom bombardment mass spectroscopy and reflectance spectra. Synthesized complexes exhibit the only d-d band at ∼
Mechanistic pathway for controlled extraction of guest molecule bound to herring sperm DNA using α-cyclodextrin.
Jaffer SS, Ghosh P, Purkayastha P.
Spectrochimica Acta Part A: Molecular Spectroscopy, 78, 1587-1591 (2011)
Synthesis, spectroscopic, crystal structure and DNA binding of Ru(II) complexes with 2-hydroxy-benzoic acid [1-(4-hydroxy-6-methyl-2-oxo-2H-pyran-3-yl)-ethylidene]-hydrazide.
Chitrapriya N, Sathiya Kamatchi T, et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 81, 128-134 (2011)

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