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CS0001

Sigma-Aldrich

Phospholipid quantification Assay Kit (Colorimetric/Fluorometric)

sufficient for 200 colorimetric or fluorometric tests

Synonym(s):

DDPC, DEPC, DLOPC, DLPC, DMPC, DOPC, DPPC, DSPC, EPC, HEPC, HSPC, LYSOPC, MPPC, MSPC, PC, PMPC, POPC, PSPC, Phosphatidylcholine, SMPC, SOPC, lecithin, lysolecithin, sphingomyelin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.84

usage

sufficient for 200 colorimetric or fluorometric tests

shipped in

dry ice

storage temp.

−20°C

General description

Phospholipids are a class of lipids, which constitute a major component of cell membranes and lipid bilayers. Phospholipids play important roles in signal transduction. The majority of phospholipids contain one diglyceride or sphingosine, a phosphate group, and one choline.

The Phospholipid quantification assay kit provides a simple and highly sensitive method for measuring choline-containing phospholipids in biological samples. This high-throughput assay can be done within only 30 minutes . In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to release choline, which is determined using choline oxidase and an H2O2 specific dye. The readout is either fluorescence or absorbance, allowing maximal flexibility and a large detection range. The linear range is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance). Phosphatidylcholine-containing phospholipids are present in serum at ~0.2-2.5 mM (~50-200 mg/dL).

The detection range of the kit is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance).

Application

  • Measuring choline-containing phospholipids in biological samples (For example: Serum and Plasma)
  • Enables evaluation of drug effects on phospholipids metabolism

Features and Benefits

Our economical Phospholipid quantification assay kit also saves your time and efforts:

  • Highly sensitive, simple and high-throughput assay for measuring choline-containing phospholipids in biological samples
  • User friendly: No need to weigh or calculate needed amounts of reagents on your own
  • Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
  • Quick instruction bench card - to assure your experimental success
  • More experiments in one kit - contains sufficient reagents for 200 tests.
  • Suitable for both colorimetric and fluorometric detection
  • Detection time: only 30 minutes

Suitability

Suitable for the detection of phospholipids in biological samples

pictograms

FlameHealth hazard
GHS02,GHS08

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

3 - Flammable liquids

flash_point_f

No data available

flash_point_c

No data available

Certificates of Analysis (COA)

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Sophie Lau et al.
Physical chemistry chemical physics : PCCP, 24(38), 23651-23660 (2022-09-23)
Cholesterol is an essential component of eukaryotic cellular membranes that regulates the order and phase behaviour of dynamic lipid bilayers. Although cholesterol performs many vital physiological roles, hypercholesterolaemia and the accumulation of cholesterol in atherosclerotic plaques can increase the risk

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