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Key Documents

C2

94101906, rat liver, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

C2, 94101906

biological source

rat liver

growth mode

Adherent

karyotype

Hyperdiploid, 52 Chromosomes

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat hepatoma [HGPRT-]

Cell Line Description

Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.

Application

Gene expression analysis.

Culture Medium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density is reached at 10x10,000,000,000 cells/10cm2 dish.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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