RAG

89040605, mouse kidney, Ameboid-like

• UNSPSC Code: 41106514

Properties

• Product Name: RAG, 89040605
• biological source: mouse kidney
• growth mode: Adherent
• karyotype: Modal no. 68, hypertriploid
• morphology: Ameboid-like
• products: ES-2
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse BALB/c renal adenocarcinoma

Cell Line Description

Derived from the original Renal-2a BALB/c renal adenocarcinoma. Cells produce the unique kidney specific esterase ES-2 and have been used in somatic cell hybridisation studies because of their azaguanine resistant properties.

Application

Hybridisation, resistance and tumourigenicity studies

Culture Medium

EMEM (HBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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