CRFK (BVD Ag negative)
86093002, feline kidney, Epithelial
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About This Item
UNSPSC Code:
41106514
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product name
CRFK (BVD Ag negative), 86093002
biological source
feline kidney
growth mode
Adherent
karyotype
Hypodiploid, modal no 36
morphology
Epithelial
products
Carcinoembryonic antigen (CEA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Cat kidney
Cell Line Description
CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.
Application
Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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