Do-11-10
85082301, mouse unknown/unspecified, Not specified
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About This Item
UNSPSC Code:
41106514
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product name
Do-11-10, from mouse unknown/unspecified, 85082301
biological source
mouse unknown/unspecified
growth mode
Suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147
Cell Line Description
Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.
Culture Medium
DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.
Other Notes
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Certificates of Analysis (COA)
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