111A3

85062504, Application: Fusion partner in the production of heterohybridomas

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Bovine Heterohybridoma
• ImmunoDon: Jersey calf lymph node
• Myeloma: 53B3 (mouse x bovine heteromyeloma)
• Reactivity: Application: Fusion partner in the production of heterohybridomas
• technique(s): cell culture | mammalian: suitable
• isotype: Not specified
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse 53B3 x Bovine Jersey Calf lymph node

Cell Line Description

Non Ig secreting

Immunogen

Sheep red blood cells

Application

This line has been used as a HAT sensitive fusion partner in the production of heterohybridomas

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Other Notes

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