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Key Documents

OX-110

13082225, from Rat x Rat Hybridoma

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About This Item

UNSPSC Code:
41106514

biological source

Rat x Rat Hybridoma

ImmunoDon

Rat DA spleen

Myeloma

Y3

Reactivity

Mouse CD200R

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Description

The hybridoma cell line OX-110 secretes monoclonal IgG2a antibodies reactive with mouse CD200R.

Immunogen

Recombinant CD200R

Application

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Seed at 5 x 104 cells/ml (5 x 105 cells/ml at resuscitation). Subculture when cells reach 5 x 105 -1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. 20% FBS can be used at resuscitation.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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