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Key Documents

htr-9c cell line

10080902, Mouse monoclonal against human CD-120a

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About This Item

UNSPSC Code:
41106514

biological source

Human x Mouse Hybridoma

ImmunoDon

Mouse (Mus musculus) spleen

Myeloma

PAI

Reactivity

Human CD120a (TNF receptor p55)

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Not specified

Cell Line Description

Mouse monoclonal against human CD-120a. TNF receptor p55 prepared from HL-60 cells (htr-mAb series).

Immunogen

Human CD120a (TNF receptor p55)

Application

FACS, Sandwich ELISA for soluble p55 TNF-R.

Culture Medium

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 25 U/ml Interleukin-6 (IL-6) + 5-10% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA maybe required to dislodge them from the plastic.

Subculture Routine

Seed cultures between 1-3 x 105 cells/ml; Saturation density 8-10 x 105 5% CO2; 37 °C.

Other Notes

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