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BCA1

Sigma-Aldrich

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

Synonym(s):

BCA protein assay

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About This Item

UNSPSC Code:
12352106
NACRES:
NA.32

Quality Level

200

usage

sufficient for ~500 assays

storage temp.

2-8°C

Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.

Features and Benefits

  • Simple, sensitive colorimetric assay for proteins
  • Two stable reagents are used for the working solution
  • Faster and easier than the Lowry protein assay
  • Compatible with many detergents both ionic and nonionic
  • Less variation between different proteins than the Bradford dye-binding assay
  • Adaptable for use in microwell plates

Principle

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Kit Components Only

Product No.
Description
  • Bicinchoninic Acid Solution 1 L
  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL
  • Protein Standard Solution 5 x 1

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tools for Protein Quantitation

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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