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A6103

Sigma-Aldrich

Aprotinin

recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

Synonym(s):

BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor

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About This Item

EC Number:
232-994-9
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)

recombinant

expressed in Nicotiana (tobacco)

Quality Level

300

description

recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

powder

specific activity

≥5 TIU/mg protein

mol wt

~_6.5 kDa

technique(s)

inhibition assay: suitable

solubility

0.85% sodium chloride: 5 mg/mL

UniProt accession no.

P00974

storage temp.

2-8°C

Gene Information

cow ... PTI(404172)

Unit Definition

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Preparation Note

Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

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