A5844

Monoclonal Anti-c-Abl antibody produced in mouse

clone ABL-148, ascites fluid

• Synonym(s): Anti-ABL, Anti-BCR-ABL, Anti-CHDSKM, Anti-JTK7, Anti-bcr/abl, Anti-c-ABL, Anti-c-ABL1, Anti-p150, Anti-v-abl
• MDL number: MFCD00239646
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: ABL-148, monoclonal
• mol wt: antigen 145 kDa
• species reactivity: monkey, rat, human, mouse, bovine
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:2,000 using human melanoma cell extract
• isotype: IgG2a
• UniProt accession no.: P00519
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ABL1(25) ; mouse ... Abl1(11350) ; rat ... Abl1(311860)

Description

General description

The c-Abl protein contains three high mobility group-like domains that bind to AT-rich DNA in a cooperative manner.

c-Abl is a proto-oncogene product that belongs to Tyr protein kinase family. It has a crucial role in different cellular processes like- differentiation, adhesion and regulates DNA damage-induced apoptosis.

Specificity

Mouse anti-c-Abl antibody reacts specifically with an epitope present in the SH2 domain of the c-Abl. The product has also shown reactivity for c-Abl of monkey, rat, bovine, mouse and human.

Immunogen

recombinant c-Abl, SH2 domain.

Application

Monoclonal anti-c-Abl antibody can be used in western blotting (diluted 1:2000) using melanoma cell extract from human. It can also be used in microarray and flow cytometry. Monoclonal anti-c-Abl antibody can be used for studying the mechanism of signaling pathways involving c-Abl. It may also be used for immunoprecipitation and immunocytochemistry.

Biochem/physiol Actions

Cytoplasmic c-Abl regulates SH2/SH3 adaptor protein cytoskeleton-associated adaptor protein (Crk) and the Crk-binding protein p130cas. Nuclear c-Abl has been implicated in the regulation of cell cycle-dependent and DNA damage-induced gene expression.

Target description

c-Abl is a non-receptor tyrosine kinase with both cytoplasmic and nuclear functions. The Abl oncogen has been implicated in several human leukemias including nearly all chronic myelocytic leukemias.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable