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A2522

Sigma-Aldrich

Actin from rabbit muscle

≥85% (SDS-PAGE), lyophilized powder

Synonym(s):

Alpha-actin 1, G-Actin

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About This Item

CAS Number:
51005-14-2
MDL number:
MFCD00130338
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rabbit

Quality Level

200

assay

≥85% (SDS-PAGE)

form

lyophilized powder

mol wt

43 kDa

composition

Protein, ≥80% biuret

technique(s)

immunoblotting: suitable

UniProt accession no.

P68135

storage temp.

2-8°C

Gene Information

rabbit ... LOC(100342017)

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General description

Actin is the abundant protein found in many cells which accounts for 10% or more of total protein. Most of the eukaryotic cells shows the presence of actin protein. Outer and inner domains are the two important domains of actin.

Application

Actin from rabbit muscle has been used:
  • to study its protective role on rabbit muscle lactate dehydrogenase from ascorbate inhibition
  • as standard in SDS gel and immunoblotting
  • in the protein pin array assays

Biochem/physiol Actions

Actin plays a key role in protein-protein interactions. It participates in cell motility, cell shape maintenance and polarity to the regulation of transcription.

Physical form

Lyophilized powder containing Tris, ATP and CaCl2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Actin and Actin-Binding Proteins
Pollard TD, et al.
Cold Spring Harbor Perspectives in Biology, 8(8) (2016)
Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat
Barash IA, et al.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 91-98 (2002)
Pooneh Vaziri et al.
Genetics, 216(1), 177-190 (2020-08-06)
Myosin is an essential motor protein, which in muscle is comprised of two molecules each of myosin heavy-chain (MHC), the essential or alkali myosin light-chain 1 (MLC1), and the regulatory myosin light-chain 2 (MLC2). It has been shown previously that MLC2
Interactive sequences in the stress protein and molecular chaperone human alphaB crystallin recognize and modulate the assembly of filaments
Ghosh JG, et al.
The International Journal of Biochemistry & Cell Biology, 39(10), 1804-1815 (2007)
J A Henderson et al.
Endocrine, 33(3), 285-293 (2008-11-21)
To further understand the role of ovarian hormones in the function of the serotonin neural system, we investigated the effects of estradiol (E), progesterone (P), and raloxifene on 5HT 1A and 2C receptor protein expression in the dorsal raphe region
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Articles

Actin

Although actin is one of the most conserved eukaryotic proteins, it is expressed as six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of the six isoforms represent differentiation markers of muscle tissues. The other two are found in practically all cells.

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