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A2055

Sigma-Aldrich

ACCUSPIN Tubes Sterile, 50 mL Capacity

radiation sterilized tube fitted with a high density polyethylene barrier

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile; irradiated

application(s)

hematology
histology

storage temp.

15-25°C

General description

ACCUSPIN Tubes are polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 15 mL of the density gradient.

Application

ACCUSPIN Tubes Sterile, 50 mL Capacity is intended for use with the density gradient separation medium Histopaque™ -1077:


  • in the isolation of lymphocytes and other mononuclear cells.
  • isolation of cord blood–derived endothelial progenitor cells.
  • isolate mononuclear cell layer from bone marrow samples.
  • suitable for the purification of human bone marrow aspirates for the investigation of the therapeutic value of BET inhibitors for acute myeloid leukemia.

Principle

Following the addition of Histopaque-1077 to theACCUSPIN Tube, a brief centrifugation places theHistopaque-1077 below the frit. The blood sample canbe added to the top chamber of the tube without risk ofmixing with the Histopaque-1077 in the lower chamberunder the frit. On subsequent centrifugation the wholeblood descends through the frit to contact with theHistopaque-1077. The elements of greater densitydisplace a volume of Histopaque-1077 above the fritgiving a clear separation of the blood components. Theerythrocytes aggregate and the granulocytes becomeslightly hypertonic, increasing their sedimentation rate,resulting in pelleting at the bottom of the ACCUSPINTube. Lymphocytes and other mononuclear cells, e.g.,monocytes, remain at the plasma/Histopaque-1077interface. This dense band of mononuclear cells maybe collected by pouring off the contents of the upperchamber or by means of a pipette. Erythrocytecontamination is avoided due to the barrier between thechambers.

Quantity

Package contains 10 tubes

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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S A Mireku et al.
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Bacterial ABC importers catalyze the uptake of essential nutrients including transition metals and metal-containing co-factors. Recently, an IgG antibody targeting the external binding protein of the Staphylococcus aureus Mn(II) ABC importer was reported to inhibit transport activity and reduce bacterial
Kara S Cox et al.
mAbs, 8(1), 129-140 (2015-10-23)
Monitoring antigen-specific memory B cells and the antibodies they encode is important for understanding the specificity, breadth and duration of immune response to an infection or vaccination. The antibodies isolated could further help design vaccine antigens for raising relevant protective
Aimin Tang et al.
Nature communications, 10(1), 4153-4153 (2019-09-14)
Respiratory syncytial virus (RSV) infection is the leading cause of hospitalization and infant mortality under six months of age worldwide; therefore, the prevention of RSV infection in all infants represents a significant unmet medical need. Here we report the isolation
Juan M Melero-Martin et al.
Methods in enzymology, 445, 303-329 (2008-11-22)
Rapid and complete vascularization of ischemic tissues and thick engineered tissues is likely to require vasculogenesis. Therefore, the search for clinically relevant sources of vasculogenic cells and the subsequent development of experimental models of vasculogenesis is of utmost importance. Here
Xiao Xiao et al.
mAbs, 11(8), 1415-1427 (2019-08-14)
Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract infection in young children and older adults. Currently, no licensed vaccine is available, and therapeutic options are limited. The primary target of neutralizing antibodies to RSV is the

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