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94281

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

powder, white, ~8 U/mg

Synonym(s):

Jack bean urease, Urea amidohydrolase

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About This Item

CAS Number:
9002-13-5
Enzyme Commission number:
3.5.1.5 (BRENDA, IUBMB)
EC Number:
232-656-0
MDL number:
MFCD00070858
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Canavalia ensiformis

Quality Level

100

form

powder

specific activity

~8 U/mg

mol wt

Mr ~480000

color

white

storage temp.

−20°C

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General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH. Urease is present in archaea, bacteria, unicellular eukaryotes and plants.

Application

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25°C

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

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Isothermal titration calorimetry to characterize enzymatic reactions
Test, 567, 215-236 (2016)
Yersinia enterocolitica
Molecular Medical Microbiology, 1319-1344 (2015)
Alfonso Muñoz et al.
Journal of experimental botany, 62(1), 307-318 (2010-09-04)
Ureidoglycolate is an intermediate in the degradation of the ureides, allantoin and allantoate, found in many organisms. In some leguminous plant species these compounds are used to transport recently fixed nitrogen in the root nodules to the aerial parts of
Nucleic acids research, 40(Database issue), D71-D75 (2011-11-22)
The mission of UniProt is to support biological research by providing a freely accessible, stable, comprehensive, fully classified, richly and accurately annotated protein sequence knowledgebase, with extensive cross-references and querying interfaces. UniProt is comprised of four major components, each optimized
Nizam Uddin et al.
Fitoterapia, 84, 202-207 (2012-10-30)
Seven constituents were isolated from the stems of Lawsonia alba Lam., following an activity-guided isolation, which include two new constituents, namely lawsorosemarinol (1) and lawsofructose (2), one known compound 2-(β-d-glucopyranosyloxy)-1, 4-naphthoquinone (3) and four compounds, 4-hydroxy coumarine (4), 3-(4-hyroxyphenyl)-triacontyl-(Z)-propenoate (5)
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