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Sigma-Aldrich

5-Carboxyfluorescein

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(s):

5-FAM

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About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
76823-03-5
Molecular Weight:
376.32
Beilstein/REAXYS Number:
57037
MDL number:
MFCD00036874
UNSPSC Code:
12171500
PubChem Substance ID:
57653195
NACRES:
NA.32

product line

BioReagent

assay

≥95% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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The loss-of-allele assay for ES cell screening and mouse genotyping
Methods in Enzymology, 476, 295-307 (2010)
Intracellular pH and its Measurement (1989)
Detection of Bacteria, Viruses, Parasites and Fungi (2010)
Study on leakage of sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes
Hudiyanti D, et al.
Orient. J. Chem., 31, 435-439 (2015)
M Goetz et al.
Endoscopy, 39(4), 350-356 (2007-04-12)
Although various improvements in tissue imaging modalities have recently been achieved, in-vivo molecular and subsurface imaging in the field of gastroenterology remains a technical challenge. In this study we evaluated a newly developed, handheld, miniaturized confocal laser microscopy probe for
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