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Sigma-Aldrich

Phosphatase, Alkaline from calf intestinal mucosa

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Synonym(s):

Alkaline Phosphatase from calf intestinal mucosa

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About This Item

CAS Number:
9001-78-9
Enzyme Commission number:
3.1.3.1 (BRENDA, IUBMB)
EC Number:
232-631-4
MDL number:
MFCD00131849
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54

biological source

bovine (calf) intestinal mucosa

form

solution (clear, colorless)

specific activity

~2500 U/mg protein (~10 mg/ml)

mol wt

Mr ~140000

technique(s)

enzyme immunoassay: suitable

impurities

0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine

pH

~7.6

storage temp.

2-8°C

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Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Reconstitution

The solution can be used without dialysis (for enzyme immuno-assay).

Other Notes

Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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M.I. Halliday et al.
Biochemical Society Transactions, 14, 473-473 (1986)
K.R. Wehrmeyer et al.
Journal of Liquid Chromatography, 6, 2141-2141 (1983)
D G Williams
Journal of immunological methods, 72(1), 261-268 (1984-08-03)
One of the requirements for enzyme immunoassay is the formation of a labelled component of the assay system which can be either the ligand or the binder. Three methods for conjugating alkaline phosphatase to human placental lactogen were investigated, involving
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
E Engvall et al.
Journal of immunology (Baltimore, Md. : 1950), 109(1), 129-135 (1972-07-01)
C K Kim et al.
Journal of immunological methods, 159(1-2), 101-106 (1993-02-26)
Immunoliposomes were prepared and their immunoassay applications investigated. Liposomes were prepared from cholesterol and phospholipids including maleimidobenzoylphosphatidylethanolamine (MBPE) for conjunction with thiol-containing antigens. Alkaline phosphatase (APase) was entrapped in the liposome and BSA, the antigen, was modified by reaction with
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