68360

Fluorescent Red Mega 485 NHS-ester

BioReagent, suitable for fluorescence, ≥90% (coupling to amines)

• Empirical Formula (Hill Notation): C₂₉H₃₃N₃O₉S
• CAS Number: 890317-36-9
• Molecular Weight: 599.65
• MDL number: MFCD06798200
• UNSPSC Code: 12352108
• PubChem Substance ID: 329760862
• NACRES: NA.32

Properties

• product line: BioReagent
• assay: ≥90% (coupling to amines)
• form: powder
• fluorescence: λ_ex 482 nm; λ_em 559 nm in 0.1 M phosphate pH 7.0
• suitability: suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: CCN(CCCCCC(=O)ON1C(=O)CCC1=O)c2ccc3C=C(C(=O)Oc3c2)c4cc[n+](CCCS([O-])(=O)=O)cc4
• InChI: 1S/C29H33N3O9S/c1-2-31(15-5-3-4-7-28(35)41-32-26(33)10-11-27(32)34)23-9-8-22-19-24(29(36)40-25(22)20-23)21-12-16-30(17-13-21)14-6-18-42(37,38)39/h8-9,12-13,16-17,19-20H,2-7,10-11,14-15,18H2,1H3
• InChI key: VKRRBXLGPVGMGA-UHFFFAOYSA-N

Description

Application

Fluorescent Red Mega 485 NHS-ester is an amine conjugating fluorescent biolabel that can be used for covalent coupling to proteins and other biomolecules containing primary amino groups.Fluorescent red Mega 485 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This means that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy.

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H318
• pcodes: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable