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12023C

Sigma-Aldrich

Newborn Bovine Serum

New Zealand origin

Synonym(s):

NBCS, NBS, NCS, calf sera, calf serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine (calf)

Quality Level

500

sterility

sterile-filtered

origin

New Zealand origin

technique(s)

cell culture | mammalian: suitable

impurities

≤35 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Serum is mostly required as a supplement to cell culture media. It contains macromolecules, carrier proteins, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors.
Newborn Bovine Serum (NBS) is collected from calves that are less than 10 days old. Although NBS is not as nutritionally rich as Fetal Bovine Serum (FBS), it can serve as a cost-effective substitute for FBS in many applications.

Biochem/physiol Actions

Newborn Calf Serum has been used in FACS (fluorescence-activated cell sorting) study for resuspension and dilution of cells.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, mycoplasma, and hemoglobin content
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae.
Tettelin H et al.
Proceedings of the National Academy of Sciences of the USA, 99, 12391-12391 (2002)
Alternatives to the use of fetal bovine serum: serum-free cell culture.
Gstraunthaler G
ALTEX, 20, 275-275 (2003)
Ratnasekhar Ch et al.
Nature communications, 12(1), 377-377 (2021-01-17)
Circadian clocks coordinate mammalian behavior and physiology enabling organisms to anticipate 24-hour cycles. Transcription-translation feedback loops are thought to drive these clocks in most of mammalian cells. However, red blood cells (RBCs), which do not contain a nucleus, and cannot

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