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Millipore

Selenite Cystine Broth

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

L-cystine, 0.01 g/L
lactose, 4 g/L
mixed peptone, 5 g/L
sodium dihydrogen phosphate, 10 g/L
sodium hydrogen selenite, 4 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
sample preparation

microbiology

suitability

selective for Proteus spp.
selective for Pseudomonas spp.
selective for Salmonella spp.

General description

Selenite Cystine Broth can be used for the enrichment of Salmonella from feces, food and other material.

Application

for the enrichment of Salmonella from feces, food and other material

Preparation Note

Dissolve 23 g in 1 litre distilled water. Warm carefully but not above 60°C. Do NOT sterilize. Pour into sterile containers. Sterile filter for longer storage. Store in the dark at 4°C. Discard on formation of a sediment or when the solution turns reddish.

Other Notes

Studies on the detection of Salmonella and Shigella in stool culture

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Acute Tox. 2 Oral - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - STOT RE 2

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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The Wampole Bactigen Salmonella-Shigella Latex Agglutination Test (SSLA) (Wampole Laboratories, Cranbury, New Jersey) was evaluated as a possible substitute for blind subculture of selenite broths from stool cultures. Recovery rates of Salmonella and Shigella from eosin-methylene blue (EMB) agar were

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