74303
Legionella Agar Base
suitable for microbiology, NutriSelect® Plus
Synonym(s):
BCYE Agar without L-cysteine, Base
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About This Item
UNSPSC Code:
41171607
NACRES:
NA.85
Recommended Products
sterility
non-sterile
Quality Level
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 13 g/L
charcoal activated, 2 g/L
yeast extract, 10 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
6.9±0.2 (25 °C)
application(s)
clinical testing
environmental
food and beverages
water monitoring
microbiology
suitability
nonselective for Legionella spp.
Related Categories
Application
Legionella Agar Base with the addition of supplement is used for the presumptive identification of Legionella species.
Preparation Note
Suspend 12.5 grams in 450 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add contents of 1 vial of Legionella BCYE Supplement without L-Cysteine (Cat. No. 43753).
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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A W Pasculle et al.
The Journal of infectious diseases, 141(6), 727-732 (1980-06-01)
Pittsburgh pneumonia agent (PPA) was recently cultivated from infected egg yold on charcoal yeast extract agar. PPA has now been isolated both from infected egg yolk and human lung tissue on charcoal yeast extract agar and on a new medium
P H Edelstein
Journal of clinical microbiology, 14(3), 298-303 (1981-09-01)
Eighteen types of media, all of which used charcoal yeast extract medium as a base, were tested for their ability to support the growth of a stock strain of Legionella pneumophila. Fifteen of these contained antimicrobial agents. Five antibiotic-containing media
J C Feeley et al.
Journal of clinical microbiology, 8(3), 320-325 (1978-09-01)
Yolk sac suspensions infected with the Legionnaires disease bacterium (LDB) were plated onto 17 different bacteriological agar media. The LDB grew only on Mueller-Hinton agar supplemented with 1% Iso Vitale X and 1% hemoglobin (MH-IH). This medium was subsequently analyzed
J C Feeley et al.
Journal of clinical microbiology, 10(4), 437-441 (1979-10-01)
Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as
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