09689

Ammonium acetate

BioUltra, for molecular biology, ≥99.0%

• Linear Formula: CH₃CO₂NH₄
• CAS Number: 631-61-8
• Molecular Weight: 77.08
• Beilstein/REAXYS Number: 4186741
• EC Number: 211-162-9
• MDL number: MFCD00013066
• UNSPSC Code: 12352302
• eCl@ss: 39021908
• PubChem Substance ID: 329748800
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• assay: ≥99.0% (calc. based on dry substance, T); ≥99.0%
• form: solid
• impurities: insoluble matter, passes filter test; ≤0.005% KMnO₄-reducing substances; ≤2% water
• ign. residue: ≤0.01% (as SO₄)
• pH: 6.5-7.5 (25 °C, 1 M in H₂O)
• mp: 110-112 °C (dec.) (lit.)
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤5 mg/kg; nitrate (NO₃^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤2 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.015; λ: 280 nm A_max: ≤0.010
• SMILES string: N.CC(O)=O
• foreign activity: DNAse, none detected; Phosphatase, none detected; Protease, none detected; RNAse, none detected
• InChI: 1S/C2H4O2.H3N/c1-2(3)4;/h1H3,(H,3,4);1H3
• InChI key: USFZMSVCRYTOJT-UHFFFAOYSA-N

Description

Application

• Modified QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry for detection of cyclopiazonic acid in feeds.: Ammonium acetate is utilized in a modified QuEChERS extraction method, improving the detection of mycotoxins in animal feed and contributing to agricultural safety and animal health (Peng et al., 2024).


• Simultaneous determination of 31 banned veterinary drugs during egg-laying period in poultry eggs by ultra performance liquid chromatography-tandem mass spectrometry.: Highlights the role of ammonium acetate in a sensitive analytical method for monitoring drug residues in poultry eggs, crucial for food safety and consumer protection (Zhu et al., 2024).


• Evaluation of diethyl 4-(5-bromo-1H-indol-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate: synthesis, anti-corrosion potential, and biomedical applications.: Discusses the use of ammonium acetate in the synthesis and evaluation of novel compounds with applications in materials science and medicine, showcasing its versatility in chemical synthesis (Ahamed et al., 2024).


• Copper(II)/polyimide linked covalent organic framework as a powerful catalyst for the solvent-free microwave irradiation-based synthesis of 2,4,5-trisubstituted imidazoles.: Features the application of ammonium acetate in the development of advanced catalytic systems for green chemistry, promoting sustainable manufacturing processes (Sedaghat et al., 2023).

Biochem/physiol Actions

Ammonium acetate can be used in x-ray crystallography, optimization reagents, protein structural analysis, and proteomics. It can also be used in a study to develop a novel and simple high-performance liquid chromatography (HPLC) method for the simultaneous determination of two selective serotonin reuptake inhibitors and two serotonin-norepinephrine reuptake inhibitors in alternative samples of toxicological interest such as hair, nail clippings, and cerebrospinal fluid.

Other Notes

Elution buffer in separation of proteins by gel filtration, ammonium acetate is volatile and can be removed during lyophilization.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)