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Roche

DNA Molecular Weight Marker VII, DIG-labeled

greener alternative

Synonym(s):

DNA marker

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About This Item

UNSPSC Code:
41105335

form

solution

Quality Level

packaging

pkg of 500 μL (10 μg/ml)

manufacturer/tradename

Roche

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

10 μg/mL

color

colorless

solubility

water: miscible

greener alternative category

storage temp.

−20°C

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
DNA Molecular Weight Marker VII, DIG-labeled is a ready-to-use solution in 10mM Tris-HCl, 1mM EDTA, pH 8.0. The fragment mixture is prepared by cleavage of SPP1 DNA with EcoRI. the size ranges from 0.081 to 8.57kbp.

Application

DNA Molecular Weight Marker VII, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.

Sequence

The mixture contains 17 fragments wit the following base pair lengths: 81, 359, 492, 710, 718, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 bp.

Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

No data available

flash_point_c

No data available


Certificates of Analysis (COA)

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Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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