11089161001
Roche
Streptavidin-AP conjugate
solution, pkg of 1 mL (1,000U)
Synonym(s):
streptavidin
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About This Item
UNSPSC Code:
12352200
Recommended Products
form
solution
Quality Level
packaging
pkg of 1 mL (1,000U)
manufacturer/tradename
Roche
storage temp.
2-8°C
General description
Streptavidin is covalently coupled to alkaline phosphatase (AP) for the preparation of streptavidin-AP-conjugate.
Alkaline Phosphatase
Alkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product.
Alkaline Phosphatase
Alkaline Phosphatase is often used as a label in enzyme immunoassays. AP converts the sensitive substrate X-phosphate/NBT into a stable, water soluble product.
Application
Streptavidin-AP has been used to detect biotinylated compounds using:
- ELISA
- Immunohistocytochemistry
- Western blot
Physical form
Solution, stablilized in 50 mM Triethanolamine buffer, 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 10 mg/ml bovine serum albumin, pH 7.6.
Preparation Note
Working solution: 50 - 1,000 mU/ml (i.e., stock solution can be diluted). Dilute the conjugate stock solution in 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline.
ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution)
Immunohisto/cytochemistry: 250 - 1,000 mU/ml (1:1000 - 1:4000 dilution)
Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution)
Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze!
Working concentration: Working concentration of conjugate will depend on the application and substrate. Indicated concentrations should be taken as a guideline.
ELISA: 50 - 200 mU/ml (1:5000 - 1:20,000 dilution)
Immunohisto/cytochemistry: 250 - 1,000 mU/ml (1:1000 - 1:4000 dilution)
Western blot: 200 - 500 mU/ml (1:2000 - 1:5000 dilution)
Stability: The concentrated stock solution is stable when stored at 2 to 8°C until the expiration date printed on the label. Do not freeze!
Other Notes
For life science research only. Not for use in diagnostic procedures.
Related product
Product No.
Description
Pricing
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
No data available
flash_point_c
No data available
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