10884308001

CPRG

Chlorophenol red-β-D-galactopyranoside

• Synonym(s): Chlorophenol Red-β-D-galactopyranoside, chlorophenol red-β-d-galactopyranoside, CPRG, Chlorophenol Red-β-D-galactoside
• Empirical Formula (Hill Notation): C₂₅H₂₂Cl₂O₁₀S
• CAS Number: 99792-79-7
• Molecular Weight: 585.41
• MDL number: MFCD05863726
• UNSPSC Code: 12352204
• PubChem Substance ID: 329749219

Properties

• Quality Level: 100
• assay: 96% (HPLC)
• form: powder
• packaging: pkg of 250 mg
• manufacturer/tradename: Roche
• storage condition: protect from light
• pH range: 6-8
• shipped in: dry ice
• storage temp.: −20°C (−15°C to −25°C)
• SMILES string: OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
• InChI key: YOUWVNCQJOMMEU-AETRGLENSA-N

Description

General description

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength dye which is commonly used for colorimetric assays. The reaction product has a dark red color and is soluble in water. It can be evaluated photometrically at 574 or 578 nm.
The chemical formula on the label represents the monosodium salt.

Application

CPRG has been used as a substrate for β-galactosidase assay.

Colorimetric determination of β-Gal activity in cell extracts and ELISA.

Biochem/physiol Actions

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

Quality

Contaminant: <0.1% chlorophenol red

Sequence

CPRG is an approximately 1:1 mixture of the isomers.

Preparation Note

Working concentration: 5 mmol/l
Storage conditions (working solution): Reconstituted solution is stable for one day at 2 to 8 °C

Storage and Stability

Store powder dry at -15 to -25 °C; stock solutions at 2–8 °C protected from light.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available