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10884308001

Roche

CPRG

Chlorophenol red-β-D-galactopyranoside

Synonym(s):

Chlorophenol Red-β-D-galactopyranoside, chlorophenol red-β-d-galactopyranoside, CPRG, Chlorophenol Red-β-D-galactoside

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About This Item

Empirical Formula (Hill Notation):
C25H22Cl2O10S
CAS Number:
99792-79-7
Molecular Weight:
585.41
MDL number:
MFCD05863726
UNSPSC Code:
12352204
PubChem Substance ID:
329749219

Quality Level

100

assay

96% (HPLC)

form

powder

packaging

pkg of 250 mg

manufacturer/tradename

Roche

storage condition

protect from light

pH range

6-8

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

SMILES string

OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1

InChI key

YOUWVNCQJOMMEU-AETRGLENSA-N

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General description

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength dye which is commonly used for colorimetric assays. The reaction product has a dark red color and is soluble in water. It can be evaluated photometrically at 574 or 578 nm.
The chemical formula on the label represents the monosodium salt.

Application

CPRG has been used as a substrate for β-galactosidase assay.
Colorimetric determination of β-Gal activity in cell extracts and ELISA.

Biochem/physiol Actions

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

Quality

Contaminant: <0.1% chlorophenol red

Sequence

CPRG is an approximately 1:1 mixture of the isomers.

Preparation Note

Working concentration: 5 mmol/l
Storage conditions (working solution): Reconstituted solution is stable for one day at 2 to 8 °C

Storage and Stability

Store powder dry at -15 to -25 °C; stock solutions at 2–8 °C protected from light.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Matthias A Fügi et al.
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Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 6(5), 375-380 (2001-01-01)
The finding that a variety of chemicals display oestrogenic activity has resulted in the development of in vitro and in vivo assays to assess oestrogenic activity. One such assay, the yeast oestrogen assay (YES) makes use of recombinant yeast cells
Evan Amalfitano et al.
Nature communications, 12(1), 724-724 (2021-02-03)
Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a
Clémence Sicard et al.
Analytical and bioanalytical chemistry, 406(22), 5395-5403 (2014-06-29)
We report on a new fluorimetric assay for β-galactosidase (β-gal) and faecal coliform bacteria that utilizes a long-wavelength dye, chlorophenol red-β-D-galactopyranoside (CPRG), that has been widely used for colorimetric assays. The novel feature of this new assay is the unexpected

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