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Key Documents

ST1718

Sigma-Aldrich

Anti-SMO Mouse mAb (1D9)

liquid, clone 1D9, Calbiochem®

Synonym(s):

Anti-Smoothened

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

1D9, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SMO(6608)

General description

Purified mouse monoclonal antibody. Recognizes the ~75 kDa SMO protein.
Recognizes the ~75 kDa SMO protein in A431 cells.
This Anti-SMO Mouse mAb (1D9) is validated for use in Immunoblotting for the detection of SMO.

Immunogen

Full-length, recombinant, human SMO (aa 653-787) expressed as a GST fusion protein

Application


Immunoblotting (1-5 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
A431 cells

Other Notes

Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Silvia Pietrobono et al.
Cell death & disease, 9(2), 142-142 (2018-02-06)
Aberrant activation of the Hedgehog (HH) signaling is a critical driver in tumorigenesis. The Smoothened (SMO) receptor is one of the major upstream transducers of the HH pathway and a target for the development of anticancer agents. The SMO inhibitor

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