RTHYMAG-30K

MILLIPLEX^® Rat Thyroid Magnetic Bead Panel - Endocrine Multiplex Assay

The analytes available for this multiplex kit are: TSH, T3, T4.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• Quality Level: 200
• species reactivity: rat
• manufacturer/tradename: Milliplex^®
• assay range: sensitivity: 2255 pg/mL; (T4); sensitivity: 284 pg/mL; (T3); sensitivity: 38 pg/mL; (TSH); standard curve range: 41-10,000 pg/mL; (TSH & T3); standard curve range: 823-200,00 pg/mL; (T4); inter-assay cv: <15%; intra-assay cv: <10%
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

The synthesis and release of Triiodothyronine (T3) and thyroxine (T4) are regulated by Thyroid-Stimulating Hormone (TSH), which is secreted by the anterior pituitary gland. These thyroid hormones play critical roles in the regulation of energy balance, metabolism of carbohydrates, fatty acids and proteins. Thyroid hormones are critical to the development of the central nervous and skeletal systems. The simultaneous monitoring of all three hormones can be of great value when researching disease models and therapeutic response.

MILLIPLEX^® Rat Thyroid Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture samples: T3 (competitive assay), T4 (competitive assay), and TSH. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Endocrine

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the analytes within the panel.

Application

• Analytes: T3, T4, TSH
• NOTE: T3 and T4 are competitive immunoassays
• Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants or lysates
• Recommended Sample Dilution: 25 μL per well 1:6 diluted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Endocrine
• Research Category: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H302 + H332,H311,H317,H319,H411
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects