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MABT117

Sigma-Aldrich

Anti-Endoglin (CD105) Antibody, clone 44G4

clone 44G4, from mouse

Synonym(s):

Endoglin, CD104

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

44G4, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

NP_000109

UniProt accession no.

P17813

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ENG(2022)

General description

Endoglin (CD105) is a homodimeric integral membrane glycoprotein comprised of a 95 kDa subunit. Endoglin is a component of the TGF-β receptor system that functions as an ancillary receptor influencing binding of the Transforming Growth Factor beta (TGF-β) family of ligands to signaling receptors. Endoglin binds to TGF-β1 and TGF-β3 with high affinity, but does not bind TGF-β2. Endoglin is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta and is weakly expressed by stromalfibroblasts. Endoglin is also expressed by U937 cells and by activated macrophages. Endoglin expression is up-regulated on activated endothelium in tissues undergoing angiogenesis, such as in tumors, or in cases of wound healing or dermal inflammation.

Immunogen

Whole HOON Pre-B Leukemic Cells

Application

Anti-Endoglin (CD105) Antibody, clone 44G4 is an antibody against Endoglin (CD105) for use in FC, IH & IP.
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory to detect Endoglin in normal human lymph node, tonsil, spleen thrymus, liver, breast, lung, and kidney specimens (Gougos, A., and Letarte, M. (1988). J Immunol. 141(6):1925-1933).

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to IP Endoglin from HOON, NALM-6, 3104, 3161, HL-60, U-937, EM-3 and KG-1a cells (Gougos, A., and Letarte, M. (1988). J Immunol. 141(6):1925-1933).
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Flow Cytometry in HUVEC cells.

Flow Cytometry Analysis: A 1:200 dilution of this antibody detected Endoglin in HUVEC cells.

Target description

71 kDa calculated

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HUVEC cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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