MABT108

Anti-TNF-alpha induced protein 6 (TSG-6) Antibody, clone NG3

clone NG3, from mouse

• Synonym(s): Tumor necrosis factor-inducible gene 6 protein, TNF-stimulated gene 6 protein, TSG-6, Tumor necrosis factor alpha-induced protein 6, TNF alpha-induced protein 6
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: NG3, monoclonal
• species reactivity: human, mouse
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_033424
• UniProt accession no.: O08859
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

Tumor Necrosis Factor-alpha (TNF-alpha), also known as TSG-6, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. The mature form of human TNF-alpha has 157 amino acid residues. TNF-alpha induced protein 6 may be involved in cell-cell and cell-matrix interactions during normal inflammatory responses and during tumorigenesis.

Specificity

Published to react with human TSG-6 (Nagyeri, G., et al. (2011). JBC. PMID: 21566135).

Immunogen

Epitope: Unknown

GST-tagged recombinant protein corresponding to mouse TSG-6.

Application

Detect TNF-alpha induced protein 6 (TSG-6) using this Anti-TNF alpha Antibody, clone NG3 validated for use in WB, ELISA. This TNF alpha antibody has been tested in Human and Mouse.

ELISA: A previous lot of this antibody was used in capture ELISA to demonstrate a typical standard curve for measuring TSG6 from normal (naïve) serum.

Western Blot Analysis: A previous lot of this antibody was show to detect human or mouse samples (Nagyeri, G., et al. (2011). JBC. PMID: 21566135).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Quality

Evaluated by Western Blot in mouse ovary tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected TSG-6 in 10 µg of mouse ovary tissue lysate.

Target description

~29 kDa observed.
An uncharacterized band appears at ~50 kDa in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse ovary tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable