MABS62

Anti-GREB1 Antibody, clone GREB1Ab

clone GREB1Ab, from mouse

• Synonym(s): growth regulation by estrogen in breast cancer 1, Gene regulated in breast cancer 1 protein, protein GREB1, gene regulated by estrogen in breast cancer protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: GREB1Ab, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_055483
• UniProt accession no.: Q4ZG55
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... GREB1(9687)

Description

General description

GREB1, also known as gene regulated in breast cancer protein 1 is shown to be involved in the proliferation of breast cancer cells, controlled by estrogen. GREB1 has the potential to be a clinical marker for response to endocrine therapy and a therapeutic target. GREB1 also may be the regulator for prostate cancer.

Immunogen

Recombinant protein corresponding to human GREB1.

Application

Immunohistochemistry Analysis: 1:600 dilution from a previous lot detected GREB1 in prostate tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IP. (Hnatyszyn, H., et al. 2009). Breast Cancer Res Treat
DOI 10.1007/s10549-009-0584-x.)

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
Hormones & Receptors

This Anti-GREB1 Antibody, clone GREB1Ab is validated for use in WB, IH, IC for the detection of GREB1.

Quality

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected GREB1 on 10 µg of MCF-7 cell lysate.

Target description

~ 216 kDa observed. Uncharacterized bands may be observed in some lysates.

Physical form

Format: Purified

Protein G

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF-7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable