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Key Documents

MABS499

Sigma-Aldrich

Anti-Phosphothreonine Antibody, clone RM102

clone RM102, from rabbit, purified by affinity chromatography

Synonym(s):

p-Thr, pthr, phospho-threonine

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

RM102, monoclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

all

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

phosphorylation (pThr)

General description

Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.

Immunogen

Mix of recombinant protein and peptide corresponding to All Phosphothreonine.

Application

Anti-Phosphothreonine, clone RM102 is an antibody against Phosphothreonine for use in WB, ICC, IH, IP.
Immunocytochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on Calyculin A/Okadaic Acid treated A431 cells
Immunohistochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on human colon cancer tissue
Immunoprecipitation Analysis: A representative lot of this antibody at 1:500 dilution immunoprecipitated phosphothreonine modified proteins from Calyculin A/Okadaic Acid treated A431 cell lysate

Quality

Evaluated by Western Blotting on Calyculin A/Okadaic Acid treated HeLa cell lysate

Western Blotting Analysis: A 1:2000 dilution of this antibody detected phosphothreonine modification on Calyculin A/Okadaic Acid treated HeLa cell lysate

Target description

N/A kDa observed

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yukika Saga et al.
Genes to cells : devoted to molecular & cellular mechanisms, 24(6), 422-435 (2019-04-20)
Dictyostelium STATa is a homologue of metazoan signal transducers and activators of transcription (STATs) and is important for morphogenesis. STATa is activated by phosphorylation on Tyr702 when cells are exposed to extracellular cAMP. Although two tyrosine kinase-like (TKL) proteins, Pyk2

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