MABS186

Anti-LOX-1, clone 9E12.1

clone 9E12.1, from mouse

• Synonym(s): LOX-1, Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1, Lectin-like oxLDL receptor 1, hLOX-1, Lectin-type oxidized LDL receptor 1, Oxidized low-density lipoprotei
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9E12.1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001166103
• UniProt accession no.: P78380
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... OLR1(4973)

Description

General description

LOX1 is a plasma membrane receptor, that also has a free soluble receptor form, that is the receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. LOX1 is also the receptor for HSP70, and LOX1 acts as a leukocyte adhesion molecule on glycosylated proteins present on platelets, monocytes, bacteria, apoptotic cells in inflammatory responses.

Immunogen

GST-tagged recombinant protein corresponding to human LOX-1.

Application

Anti-LOX-1 Antibody, clone 9E12.1 is an antibody against LOX-1 for use in Immunohistochemistry and Immunocytochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LOX-1 in human placenta tissue.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blotting in KG-1 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LOX-1 in 200 µg of KG-1 cell lysate.

Target description

~35 kDa observed. The calculated molecular weight of this protein is 31 kDa, but can be observed at ~33-37 kDa due to high glycosylation.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable