MABN70

Anti-Slo1 Antibody, clone L6/60

clone L6/60, from mouse

• Synonym(s): potassium large conductance calcium-activated channel, subfamily M, alpha member 1, Slo homolog, stretch-activated Kca channel, Maxi K channel, Drosophila slowpoke-like, Calcium-activated potassium channel, subfamily M subunit alpha-1, Slowpoke homolog,
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: L6/60, monoclonal
• species reactivity: rat
• species reactivity (predicted by homology): mouse (immunogen homology)
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_034740
• UniProt accession no.: Q08460
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KCNMA1(3778)

Description

General description

Slo1 (Calcium-activated potassium channel subunit alpha-1) may not only be activated by membrane depolarization but may also be by an increase in cytosolic Ca2+. The concentration of cytosolic Mg2+ also activates this potassium channel. Found to play a part in the repolarization of the membrane potential due to the fact that it diminishes the excitatory voltage in the cell.

Specificity

This antibody recognizes S9-S10 segments of Slo1.

Immunogen

Epitope: S9-S10 segments

Recombinant protein corresponding to the S9-S10 segments of mouse Slo1.

Application

Detect Slo1 using this Anti-Slo1 Antibody, clone L6/60 validated for use in IH, WB & IC.

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Slo1 in rat cerebellum tissue.

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Misonou, H., et al. (2006). J Comp Neurol. 496(3):289–302.)

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blot in rat brain membrane tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Slo1 on 10 µg of rat brain membrane tissue lysate.

Target description

~100 kDa observed

Physical form

Format: Purified

Protein G

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain membrane tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable