MABN2616

Anti-TMEM30A Antibody, clone 7F4

• Synonym(s): Cell cycle control protein 50A;CDC50A;P4-ATPase flippase complex beta subunit;Transmembrane protein 30A
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7F4, monoclonal
• mol wt: calculated mol wt 40.68 kDa; observed mol wt ~52-54 kDa
• purified by: using protein G
• species reactivity: human, mouse
• species reactivity (predicted by homology): feline, porcine, canine, monkey, horse, bovine
• packaging: antibody small pack of 100 μg
• technique(s): immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001068691
• UniProt accession no.: Q17QL5
• shipped in: 2-8°C
• target post-translational modification: unmodified
• Gene Information: bovine ... TMEM30A(505797)

Description

General description

Cell cycle control protein 50A (UniProt: Q17QL5; also known as P4-ATPase flippase complex beta subunit TMEM30A, Transmembrane protein 30A) is encoded by the TMEM30A gene (Gene ID: 505797) in bovine species. TMEM30A is a multi-pass membrane protein with two cytoplasmic domains, two transmembrane domains, and one extracellular domain. It is expressed in photoreceptor cells and is detected in retina outer segment and other retinal layers. It is a component of various P4-ATPase flippase complexes, which consists of a catalytic -subunit and an accessory β-subunit. It catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. The β-subunit may assist in binding of the phospholipid substrate. Most P4-ATPases associate with one of three accessory subunit isoforms known as CDC50A (TMEM30A), CDC50B (TMEM30B), and CDC50C (TMEM30C). TMEM30A is required for the proper folding, assembly and endoplasmic reticulum (ER) to Golgi exit of the ATP8A2:TMEM30A flippase complex. This ATP8A2:TMEM30A complex may be involved in regulation of neurite outgrowth. It is also involved in the uptake of platelet-activating factor (PAF). (Ref.: Liou, AY., et al. (2019). J. Biol. Chem. 294(17); 6809-6821; Wang, J., et al. (2018). Sci. Rep. 8: 10795).

Specificity

Clone 7F4 is a mouse monoclonal antibody that detects Cell cycle control protein 50A (TMEM30A). It targets an epitope within 9 amino acids from the N-terminal, cytoplasmic domain.

Immunogen

A linear peptide corresponging to 9 amino acids from the N-terminal region of bovine cell cycle control protein 50A (TMEM30A).

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse testis tissue lysate.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected TMEM30A in Mouse testis tissue lysate.

Tested applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected TMEM30A in Human brain, mouse brain, and mouse liver tissue lysates.

Immunoaffinity Purification: A representative lot was used in Immunoaffinity Purification of TMEM30A. (Wang, J., et al. (2018). Sci Rep. 8(1):10795).

Immunohistochemistry Applications: A representative lot detected TMEM30A in Immunohistochemistry applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16).

Western Blotting Analysis: A representative lot detected TMEM30A in Western Blotting applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16; Wang, J., et al. (2018). Sci Rep. 8(1):10795; Liou, A.Y., et al. (2019). J Biol Chem. 294(17):6809-6821).

Immunoprecipitation Analysis: A representative lot detected TMEM30A in Immunoprecipitation applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-TMEM30A, clone 7F4, Cat. No. MABN2616, is a mouse monoclonal antibody that detects TMEM30A and is tested for use in Immunohistochemistry, Immunoaffinity Purification, Immunoprecipitation, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable