MABF243
Anti-Par-2 Antibody, clone SAM11
clone SAM11, from mouse
Synonym(s):
Proteinase-activated receptor 2, PAR-2, Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SAM11, monoclonal
species reactivity
mouse, human
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... F2RL1(2150)
General description
The protein named Proteinase-activated receptor 2 (PAR2/PAR-2), or Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like-1 and encoded by the human gene F2RL1/GPR11/PAR2 is a receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Also as part of the innate and adaptive immunity response, PAR2 acts as sensor for proteolytic enzymes generated during infection and works to promote inflammation and the inflammatory response. PAR2 is also involved in vascular and airway tone and in blood pressure regulation and many other G protein coupled receptor response pathways. PAR2 is a multi-pass membrane protein that is widely expressed in most tissues except brain or skeletal muscle.
Immunogen
Epitope: N-terminus
Linear peptide corresponding to the N-terminus of human Par-2.
Application
Anti-Par-2 Antibody, clone SAM11 is an antibody against Par-2 for use in western blotting, IHC & flow cytometry.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Par-2 in human small intestine, coronary artery and aorta sections, and in human artery and vein tissues (Molino, M., et al. (1998). Arterioschler Throm Vasc Biol. 18(5):825-832.).
Flow Cytometry Analysis: Arepresentative lot from an independent laboratory detected Par-2 in Par-2 transfected COS-1 cells (Molino, M., et al. (1997). J Biol Chem. 272(17):11133-11141.).
Flow Cytometry Analysis: Arepresentative lot from an independent laboratory detected Par-2 in Par-2 transfected COS-1 cells (Molino, M., et al. (1997). J Biol Chem. 272(17):11133-11141.).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Quality
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Par-2 in 10 µg of NIH/3T3 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Par-2 in 10 µg of NIH/3T3 cell lysate.
Target description
~50 kDa observed. The calculated MW of this protein is 44 kDa However, it may be observed at higher molecular weights due to glycosylation.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Madhumita Paul et al.
FASEB bioAdvances, 1(4), 255-264 (2019-06-15)
Protease activated receptor 2 (PAR2) is a G-protein coupled receptor that contributes to prostate fibrosis and lower urinary tract symptoms (LUTS). In addition to fibrosis, aberrant smooth muscle tone in the prostate has been hypothesized to play a role. We
Endothelial cell thrombin receptors and PAR-2. Two protease-activated receptors located in a single cellular environment.
Molino, M, et al.
The Journal of Biological Chemistry, 272, 11133-11141 (1997)
Yanju Bao et al.
Molecular pain, 10, 28-28 (2014-06-03)
Bone cancer pain is currently a major clinical challenge for the management of cancer patients, and the cellular and molecular mechanisms underlying the spinal sensitization remain unclear. While several studies demonstrated the critical role of proteinase-activated receptor (PAR2) in the
Differential expression of functional protease-activated receptor-2 (PAR-2) in human vascular smooth muscle cells.
Molino, M, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 18, 825-832 (1998)
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