MABE2036

Anti-PADI2/PAD2 Antibody, clone DN2

• Synonym(s): PAD2, Peptidylarginine deiminase 2
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: DN2, monoclonal
• mol wt: observed mol wt ~80 kDa
• purified by: using protein G
• species reactivity: human
• species reactivity (predicted by homology): rabbit
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; immunohistochemistry: suitable; multiplexing: suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal
• Protein ID accession no.: N/A
• UniProt accession no.: A0A5F9DB16
• storage temp.: 2-8°C
• Gene Information: rabbit ... PADI2(100357368)

Description

Specificity

Clone DN2 is a mouse monoclonal antibody that detects Protein-arginine deiminase 2 (PADI2/PAD2). It targets an epitope within the N-terminal region.

Immunogen

Protein-arginine deiminase (PADI2/PAD2) from rabbit skeletal muscle.

Application

Quality Control Testing

Evaluated by Western Blotting with full-length, recombinant, Human PADI2/PAD2.

Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, recombinant, Human PADI2/PAD2.

Tested Applications

Immunohistochemistry Applications: A representative lot detected PADI2/PAD2 in Immunohistochemistry applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22).

Western Blotting Analysis: A representative lot detected PADI2/PAD2 in Western Blotting applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22; Bawadekar, M., et al. (2017). J Autoimmun. 80:39-47).

ELISA Analysis: A representative lot detected PADI2/PAD2 in ELISA applications (Damgaard, D., et al. (2014). J Immunol Methods.;405:15-22; Damgaard, D., et al. (2016). Rheumatology (Oxford). 55(5):918-27).

Multiplexing: A representative lot detected PADI2/PAD2 in Multiplexing applications (Vejlstrup, A., et al. (2019). J Inflamm Res. 29:12:137-144).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Protein-arginine deiminase (UniProt: A0A5F9DB16; also known as PADI2, PAD2) is encoded by the PADI2 gene (Gene ID: 100357368) in rabbit. Protein-arginine deiminases (PAD) catalyze the hydrolysis of peptidyl-arginine to peptidyl-citrulline and alter protein function. Five isoforms of PAD have been described in mammals (PAD1, 2, 3, 4, and 6) that are homologous in their catalytic site but are expressed differently in different cells and tissues and have different physiological roles. PAD2, one of the most abundant PADs has three domains: N-terminal domain (aa 1-114), a central domain (aa 116-274), and a C-terminal domain (aa 285-657). High calcium concentration is reported to be a prerequisite for PAD2 activation. A redox potential maintains its catalytic site in a reduced state. Overexpression of PAD2 has been associated with inflammatory diseases, such as rheumatoid arthritis and neurodegenerative diseases including multiple sclerosis. Higher levels of PAD2 are observed in transformed cell model of breast cancer progression with increasing tumor malignancy and in a murine models of xenograft tumors. Clone DN2 binds to an epitope in the N-terminal region and is ideally suited for quantitative ELISA. (Ref.: Wang, Y., et al. (2021). Int. J. Cancer. 148(2); 267-276; Vejlstrup, A., et al. (2019). J. Inflamm. Res. 12; 137 144; Damgaard, D., et al. (2016). Rheumatology 55(5); 918-927; Damgaard, D., et al. (2014). J. Immunol. Methods. 405; 15-22).

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable