MABE191

Anti-WHSC1/NSD2 Antibody, clone 29D1

clone 29D1, from mouse

• Synonym(s): Probable histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein, Nuclear SET domain-containing protein 2, Protein trithorax-5, Wolf-Hirschhorn syndrome candidate 1 protein
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 29D1, monoclonal
• species reactivity: human
• technique(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001035889
• UniProt accession no.: O96028
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WHSC1(7468)

Description

General description

Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1), Nuclear SET domain containing protein 2 (NSD2) is a histone lysine methyltransferase that is deregulated in a subgroup of multiple myelomas. NSD proteins have been described to serve as oncogenes; however, little is known about their targets in transcriptional repression or activation. NSD2 is overexpressed in multiple myelomas via translocation between the NSD2 locus on chromosome 4 and the immunoglobulin locus on chromosome 14. NSD2 is under the regulatory control of this immunoglobulin locus. Further research is required to elucidate its roles and mechanisms in cancer.

Immunogen

GST-tagged recombinant protein corresponding to human WHSC1/NSD2.

Application

Detect WHSC1/NSD2 using this Anti-WHSC1/NSD2 Antibody, clone 29D1 validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation.

Western Blot Analysis: 0.5-1 µg/mL from a representative lot detected WHSC1/NSD2 in 10 µg of HeLa nuclear extract and human placenta tissue lysate.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in transfected Gal4-reporter/293T cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in KMS11 cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.)

Quality

Evaluated by Western Blot in HCT116 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected WHSC1/NSD2 in 10 µg of HCT116 cell lysate.

Target description

~152 kDa observed. There are seven isoforms ranging between ~30 and ~152 kDa Different lysates may indicate different isoforms in Western Blots.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable