MABC958
Anti-TIM4/TIMD-4 Antibody, clone Kat5-18
clone Kat5-18, from hamster(Armenian)
Synonym(s):
T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, Spleen, mucin-containing, knockout of lymphotoxin protein, SMUCKLER, T-cell immunoglobulin mucin receptor 4, TIM-4, T-cell membrane protein 4, TIM4/TIMD-4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Recommended Products
biological source
hamster (Armenian)
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Kat5-18, monoclonal
species reactivity
mouse
technique(s)
flow cytometry: suitable
neutralization: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Timd4(276891)
Related Categories
General description
T-cell immunoglobulin and mucin domain-containing protein 4 (UniProt Q6U7R4; also known as Spleen mucin-containing knockout of lymphotoxin protein, T-cell immunoglobulin and mucin domain containing 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIMD-4, Smuckler) is encoded by the Timd4 (also known as B430010N18Rik, Tim4) gene (Gene ID 276891) in murine species. Phagocytes, including macrophages, target apoptotic cells for engulfment by recognizing their surface exposed phosphatidylserine (PtdSer or PS). Macrophages employ specific receptors and opsonins for PS recognition, such as Milk-fat globule epidermal growth factor 8 (MFG-E8), protein S, growth arrest-specific 6 (Gas6), and TAM family TKR (Tyro 3, Axl, and MerTK) that function as protein S and Gs6 receptors. Tim (T-cell immunoglobulin and mucin domain-containing) family proteins, stabilins, and BAI1 also directly bind PtdSer and enhance the engulfment of apoptotic cells by phagocytes. Tim4 is shown to mediate murine resident peritoneal macrophages (rpMacs) engagment of apoptotic cells, while MerTK-mediates the engulfment of tethered target cells. Tim4- or MerTK-null mutations prevent rpMac-mediated apoptotic cell engulfment. Tim4-null, but not MerTK-null, macrophages lose their ability to tether apoptotic cells. Murine Tim4 is initially produced with a signal peptide sequence (a.a. 1-22), the removal of which yields the mature Tim4 with a large extracellular region (a.a.23-279), a transmembrane domain (a.a. 280-300), and a short cytoplasmic tail (a.a. 301-343).
Immunogen
Epitope: Extracellular domain
Mouse peritoneal cells.
Application
Flow Cytometry Analysis: A representative lot detected Tim4 immunoreactivity among 61% of the resident peritoneal Tim4+ MerTK+ macrophages from C57BL/6J mice (Nishi, C., et al. (2014). Mol. Cell. Biol. 34(8):1512-1520).
Flow Cytometry Analysis: Representative lots were conjugated with biotin and detected Tim4 immunoreactivity among mouse Mac1+ peritoneal cells (Miyanishi, M., et al. (2012). Int. Immunol. 24(9):551-559; Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Neutralizing Analysis: A representative lot blocked Tim4-mediated engulfment of apoptotic CAD-/- thymocytes by murine peritoneal macrophages in a dose-dependent manner in culture (Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Neutralizing Analysis: A representative lot, when administered via i.v. injection, significantly suppressed the phagocytosis activity of F40/80+ macrophages in the thymus of CAD-/- mice following intraperitoneal dexamethasone injection to induce apoptosis in the thymus (Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Flow Cytometry Analysis: Representative lots were conjugated with biotin and detected Tim4 immunoreactivity among mouse Mac1+ peritoneal cells (Miyanishi, M., et al. (2012). Int. Immunol. 24(9):551-559; Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Neutralizing Analysis: A representative lot blocked Tim4-mediated engulfment of apoptotic CAD-/- thymocytes by murine peritoneal macrophages in a dose-dependent manner in culture (Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Neutralizing Analysis: A representative lot, when administered via i.v. injection, significantly suppressed the phagocytosis activity of F40/80+ macrophages in the thymus of CAD-/- mice following intraperitoneal dexamethasone injection to induce apoptosis in the thymus (Miyanishi, M., et al. (2007). Nature. 450(7168):435-439).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-TIM4/TIMD-4 Antibody, clone Kat5-18 is validated for use in Flow Cytometry and Neutralizing for the detection of TIM4/TIMD-4.
Quality
Evaluated by Flow Cytometry in Ba/F3-Tim4 cells overexpressing mouse Tim4.
Flow Cytometry Analysis: 0.1 µg of this antibody detected TIM4/TIMD-4 in Ba/F3-Tim4 cells overexpressing mouse Tim4.
Flow Cytometry Analysis: 0.1 µg of this antibody detected TIM4/TIMD-4 in Ba/F3-Tim4 cells overexpressing mouse Tim4.
Target description
~37 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified armenian hamster monoclonal IgG antibody in PBS without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chihiro Nishi et al.
Molecular and cellular biology, 34(8), 1512-1520 (2014-02-12)
Apoptotic cells are swiftly engulfed by macrophages to prevent the release of noxious materials from dying cells. Apoptotic cells expose phosphatidylserine (PtdSer) on their surface, and macrophages engulf them by recognizing PtdSer using specific receptors and opsonins. Here, we found
Masanori Miyanishi et al.
International immunology, 24(9), 551-559 (2012-06-23)
Phagocytes, including macrophages, recognize phosphatidylserine exposed on apoptotic cells as an "eat me" signal. Milk Fat Globule EGF Factor VIII (MFG-E8) is secreted by one subset of macrophages, whereas Tim4, a type I membrane protein, is expressed by another. These
Masanori Miyanishi et al.
Nature, 450(7168), 435-439 (2007-10-26)
In programmed cell death, a large number of cells undergo apoptosis, and are engulfed by macrophages to avoid the release of noxious materials from the dying cells. In definitive erythropoiesis, nuclei are expelled from erythroid precursor cells and are engulfed
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