MABC667
Anti-GPC3 Antibody, clone 9C2
ascites fluid, clone 9C2, from mouse
Synonym(s):
SGB, DGSX, MXR7, SDYS, SGBS, OCI-5, SGBS1, GTR2-2, Glypican-3, Intestinal protein OCI-5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
9C2, monoclonal
species reactivity
mouse, human
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GPC3(2719)
General description
Glypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA.
Immunogen
Purified recombinant fragment of human GPC3 expressed in E. Coli.
Application
Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GPC3 in human liver and kidney tissues.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells.
Optimal working dilutions must be determined by end user.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells.
Optimal working dilutions must be determined by end user.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Quality
Evaluated by Western Blotting in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates.
Target description
~66 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physical form
Protein G Purified
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates
Mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Repr. 1A
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Shiwei Han et al.
Oncotarget, 7(28), 44406-44416 (2016-06-04)
Gastric cancer is a prevalent tumor that is usually detected at an advanced metastatic stage. Currently, standard therapies are mostly ineffective. Here, we report that Glypican-3 (GPC3) is absent in invasive tumors and metastatic lymph nodes, in particular in aggressive
Mark S Cembrowski et al.
eLife, 7 (2018-10-31)
In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of
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