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Key Documents

MAB899

Sigma-Aldrich

Anti-HIV Nef Antibody, clone AG11

clone AG11, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AG11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

shipped in

wet ice

Gene Information

human ... TARBP1(6894)

Specificity

Recognizes a single 27 kD band in Western blots and is reactive against Nef in immunoprecipitation and immunofluorescent assays. The reactive epitope maps to the COOH terminus of the full length Nef protein. MAB899 does not recognize Nef from HIV-1SF2.

Application

Anti-HIV Nef Antibody, clone AG11 is an antibody against HIV Nef for use in ELISA, IF, IH, IP & WB.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Western blot (1:1000)
ELISA (1:100000) when tested against recombinant HIV-1IIIB Nef. For extensive dilution, protein containing or other stabilizing medium should be used.
Immunofluorescence
Immunoprecipitation
Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02 M PB + 0.25 M NaCl + 0.1% Na Azide, pH = 7.6

Storage and Stability

Maintain at 2° to 8°C for up to 12 months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael S McLemore et al.
Head and neck pathology, 4(2), 97-105 (2010-03-25)
Oncogenic human papillomaviruses (HPVs) are associated with oropharyngeal squamous cell carcinoma (SCC). Infection with human immunodeficiency virus (HIV) increases susceptibility to opportunistic infections and viral-promoted cancers. The prevalences of HPV, herpes simplex virus (HSV), Epstein-Barr virus (EBV), and human herpesvirus-8
R S Root-Bernstein
Medical hypotheses, 44(1), 20-27 (1995-01-01)
Several investigators have proposed that autoimmunity may be induced by idiotype-antiidiotype antibody networks. It is generally assumed that the antiidiotype is produced in response to the idiotype, and therefore that autoimmune diseases have single antigenic initiators. The theory of multiple-antigen-mediated

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