MAB8052
Anti-Adenovirus Antibody, clone 20/11
clone 20/11, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
clone
20/11, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
shipped in
wet ice
General description
Adenoviruses are 65-80 nm, non-enveloped, regular icosahedron pathogens often associated with respiratory and gastrointestinal illness as well as conjunctivitis. Well over 40 types of adenoviruses have currently been recognized. In addition to their deleterious effects, adenoviruses have been used in vaccine production and in gene therapy. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. Since they are easy to prepare, multiple quickly and efficiently, and have the ability to infect and deliver their genome (including researcher-created constructs) to the nucleus of a variety of post-mitotic cells, adenoviruses are extremely valuable in research and medical applications ranging from virus/host cell interaction to the insertion of novel genes or regulatory mechanisms into eukaryotic cellular systems, to delivery of therapeutic anti-cancer genes.
Specificity
Recognizes All 41 serotypes of Adenovirus.
Immunogen
Adeno 3.
Application
Anti-Adenovirus Antibody, clone 20/11 detects level of Adenovirus & has been published & validated for use in ELISA, FC, IF, IH, IH(P).
EIA, IFA
Immunohistochemistry: 1:200. In trypsinized (with 0.1% trypsin in Tris buffer pH 7.6 containing 0.1% Calcium Cloride; for 5-10 minutes at room temp.) paraffin embedded tissue. Do not use antigen retrieval.
Flow cytometry.
Final working dilutions and protocols must be determined by end user.
Immunohistochemistry: 1:200. In trypsinized (with 0.1% trypsin in Tris buffer pH 7.6 containing 0.1% Calcium Cloride; for 5-10 minutes at room temp.) paraffin embedded tissue. Do not use antigen retrieval.
Flow cytometry.
Final working dilutions and protocols must be determined by end user.
Research Category
Infectious Diseases
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
Infectious Diseases - Viral
Physical form
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Storage and Stability
Maintain at +2–8°C in convenient aliquots for up to 12 months. Do not store in a diluted format.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Analysis Note
Control
Adenovirus Control Slides, Catalogue Number 5009-5
Adenovirus Control Slides, Catalogue Number 5009-5
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Detection and quantitation of infectious human adenoviruses and JC polyomaviruses in water by immunofluorescence assay.
Calgua B, Barardi CR, Bofill-Mas S, Rodriguez-Manzano J, Girones R
Journal of Virological Methods null
SIV-associated myocarditis: viral and cellular correlates of inflammation severity.
Jennifer H Yearley, Christine Pearson, Angela Carville, Richard P Shannon, Keith G Mansfield
Aids Research and Human Retroviruses null
Adenovirus-mediated gene transfer to tumor cells.
Manel Cascallo, Ramon Alemany
Methods in Molecular Biology null
Coagulation factor IX mediates serotype-specific binding of species A adenoviruses to host cells.
Lenman, A; Muller, S; Nygren, MI; Frangsmyr, L; Stehle, T; Arnberg, N
Journal of virology null
Concurrent delivery of GM-CSF and B7-1 using an oncolytic adenovirus elicits potent antitumor effect.
Choi, KJ; Kim, JH; Lee, YS; Kim, J; Suh, BS; Kim, H; Cho, S; Sohn, JH; Kim, GE; Yun, CO
Gene Therapy null
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