MAB2617
Anti-Kindlin-2 Antibody, clone 3A3
clone 3A3, from mouse
Synonym(s):
Mitogen-inducible gene 2 protein, Pleckstrin homology domain-containing family C member 1, fermitin family homolog 2, kindlin 2, mitogen inducible gene 2 protein
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3A3, monoclonal
species reactivity
human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), rat (98% sequence homology), mouse (98% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FERMT2(10979)
General description
The Kindlin family is a group of focal adhesion proteins that have become a point of interest because of their ability to bind and activate integrins. Abnormalities in this ability have linked Kindlin to several diseases, both inherited and acquired, such as Kindler syndrome, cancer, and leukocyte adhesion deficiency. The isoform Kindlin-2 specifically participates in the recruitment of migfilin protein to focal adhesion sites for cell-ECM interactions, coordinates the manufacture of actin and modifications to cell shape. It is also actively involved in connecting ECM adhesion sites and the actin cytoskeleton (Shi, 2007). Kindlin-2 is pervasively expressed throughout tissue.
Specificity
The antibody recognizes Kindlin-2.
Immunogen
Epitope: Unknown
GST-tagged recombinant protein corresponding to human Kindlin-2.
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Kindlin-2 Antibody, clone 3A3 is validated for use in WB, IC for the detection of Kindlin-2.
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-2 in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Kindlin-2 in 10 µg of HeLa cell lysate.
Target description
~ 70 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jie Lin et al.
Journal of experimental & clinical cancer research : CR, 36(1), 134-134 (2017-10-04)
Kindlin-2 is a member of the focal adhesion protein family that regulates invasion and metastasis in multiple malignancies; however, little is known about the role of Kindlin-2 in hepatocellular carcinoma (HCC) progression. Immunohistochemistry was used to investigate Kindlin-2 expression in
Lihua Qi et al.
Cell death & disease, 10(12), 890-890 (2019-11-27)
Kindlin-2 plays an important role in the regulation of cardiac structure and function. Depletion of Kindlin-2 contributes to cardiac hypertrophy and progressive heart failure, however, the precise mechanisms involved in this process remain unclear. GATA4 is a critical transcription factor
Huanqing Gao et al.
JCI insight, 4(13) (2019-07-12)
Kindlin-2 regulates integrin-mediated cell adhesion to and migration on the extracellular matrix. Our recent studies demonstrate important roles of kindlin-2 in regulation of mesenchymal stem cell differentiation and skeletal development. In this study, we generated adipose tissue-specific conditional knockout of
Lihua Qi et al.
FEBS letters, 589(16), 2155-2162 (2015-07-06)
Kindlin-2, as an integrin-interacting protein, was known to be required for the maintenance of cardiac structure and function in zebrafish. However, the mechanism remains unclear. We found that Kindlin-2 interacts and colocalizes with α-actinin-2 at the Z-disc of mouse cardiac
Fan Lu et al.
Nature communications, 13(1), 2362-2362 (2022-04-30)
Talin-induced integrin binding to extracellular matrix ligands (integrin activation) is the key step to trigger many fundamental cellular processes including cell adhesion, cell migration, and spreading. Talin is widely known to use its N-terminal head domain (talin-H) to bind and
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