MAB2000

Anti-Integrin β1 Antibody, clone HB1.1

clone HB1.1, Chemicon^®, from mouse

• Synonym(s): CD29
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HB1.1, monoclonal
• species reactivity: human, pig
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• suitability: not suitable for activity/function inhibition
• NCBI accession no.: NM_002211.2; NM_033666.1; NM_033667.1; NM_033668.1; NM_033669.1; NM_133376.1
• UniProt accession no.: P05556
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGB1(3688)

Description

Specificity

Reacts with beta-1 subunit of VLA integrins

Application

Anti-Integrin β1 Antibody, clone HB1.1 detects level of Integrin β1 & has been published & validated for use in FC, IP, WB, IH.

Immunoprecipitation of VLA integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB2000.

Flow cytometry: 10 μg/mL

Western blot

Immunohistochemistry in frozen tissue samples: 5 μg/mL

Induction of cell aggregation: 10 μg/mL. MAB2000 is a potent stimulator of cell aggregation tested in U937, Jurkat and human peripheral blood lymphocytes.

MAB2000 does not affect VLA integrin-mediated cell adhesion to matrix proteins.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Linkage

Replaces: 04-1109

Physical form

Format: Purified

Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable