MAB19310
Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28
clone AF-28, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
AF-28, monoclonal
species reactivity
bovine, rat, human, pig, mouse
should not react with
guinea pig, horse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ACAN(176)
General description
Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.
Specificity
Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.
Immunogen
Epitope: N-terminus FFGVG neoepitopes
FFGVGGEEDC-KLH peptide
Application
Detect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
Inflammation & Autoimmune Mechanisms
ECM Proteins
Inflammation & Autoimmune Mechanisms
Western blot
ELISA
Optimal working dilutions must be determined by the end user.
ELISA
Optimal working dilutions must be determined by the end user.
Physical form
Format: Purified
Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.
Protein A purified
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Cartilage, neural tube, and brain tissue
Cartilage, neural tube, and brain tissue
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
MMP proteolysis of the human extracellular matrix protein aggrecan is mainly a process of normal turnover.
Struglics, A; Hansson, M
The Biochemical Journal null
Soluble recombinant neprilysin induces aggrecanase-mediated cleavage of aggrecan in cartilage explant cultures
Chevrier, A, et al
Archives of Biochemistry and Biophysics, 396, 178-186 (2001)
F A Mercuri et al.
The Journal of biological chemistry, 274(45), 32387-32395 (1999-11-05)
A recombinant human aggrecan G1-G2 fragment comprising amino acids Val(1)-Arg(656) has been expressed in Sf21 cells using a baculovirus expression system. The recombinant G1-G2 (rG1-G2) was purified to homogeneity by hyaluronan-Sepharose affinity chromatography followed by high performance liquid chromatography gel
Multiple functions of Osterix are required for bone growth and homeostasis in postnatal mice.
Zhou X, Zhang Z, Feng JQ, Dusevich VM, Sinha K, Zhang H, Darnay BG, de Crombrugghe B
Proceedings of the National Academy of Sciences of the USA null
The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging.
Maijenburg, MW; Kleijer, M; Vermeul, K; Mul, EP; van Alphen, FP; van der Schoot, CE; Voermans, C
Haematologica null
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