MAB1397Z
Anti-ICAM-1 Antibody, clone 3E2B, Azide Free
clone 3E2B, Chemicon®, from hamster(Armenian)
Synonym(s):
CD54, MAB1397
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
hamster (Armenian)
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
3E2B, monoclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Icam1(15894)
Specificity
MAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions.
Application
Anti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH.
Immunocytochemistry: Live or acetone fixed cells
ELISA
Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).
Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.
Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary
Clone 3E2B is reported to be function blocking against murine ICAM-1.
Optimal working dilutions must be determined by end user.
ELISA
Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).
Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.
Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary
Clone 3E2B is reported to be function blocking against murine ICAM-1.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Linkage
Replaces: CBL1331F
Physical form
Format: Purified
Purified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg
Storage and Stability
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules.
Johnston, S C, et al.
Journal of immunology (Baltimore, Md. : 1950), 145, 1181-1187 (1990)
Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55.
Fabry, Z, et al.
Journal of Neuroimmunology, 36, 1-11 (1992)
Reduced contact sensitivity reactions in mice treated with monoclonal antibodies to leukocyte function-associated molecule-1 and intercellular adhesion molecule-1.
Scheynius, A, et al.
Journal of immunology (Baltimore, Md. : 1950), 150, 655-663 (1993)
Dominique Promeneur et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(3), 1035-1040 (2013-01-02)
Tragically common among children in sub-Saharan Africa, cerebral malaria is characterized by rapid progression to coma and death. In this study, we used a model of cerebral malaria appearing in C57BL/6 WT mice after infection with the rodent malaria parasite
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