HMHEMAG-34K

MILLIPLEX^® Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay

The Human Metabolic Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 13 protein biomarkers in human serum, plasma and cell/tissue culture samples.

• Synonym(s): human metabolic hormone immunoassay panel, luminex human metabolic hormone multiplex assay, millipore human metabolism multiplex kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 101%; (Glucagon); accuracy: 102%; (Leptin); accuracy: 103%; (GIP); accuracy: 103%; (GLP-1 (Total)); accuracy: 105%; (Ghrelin (Active)); accuracy: 106%; (MCP-1); accuracy: 97%; (TNFα); accuracy: 99%; (C-Peptide); sensitivity: 0.5 pg/mL; (MinDC+2SD; TNF?); sensitivity: 1.1 pg/mL; (MinDC+2SD; GIP); sensitivity: 13 pg/mL; (MinDC+2SD; IL-6); sensitivity: 14 pg/mL; (MinDC+2SD; Ghrelin (Active)); sensitivity: 14 pg/mL; (MinDC+2SD; MCP-1); sensitivity: 146 pg/mL; (MinDC+2SD; PYY); sensitivity: 15 pg/mL; (MinDC+2SD; Amylin (Total)); sensitivity: 16 pg/mL; (MinDC+2SD; C-Peptide); sensitivity: 16 pg/mL; (MinDC+2SD; Glucagon); sensitivity: 160 pg/mL; (MinDC+2SD; Insulin); sensitivity: 2.4 pg/mL; (MinDC+2SD; GLP-1 (Active)); sensitivity: 2.9 pg/mL; (MinDC+2SD; PP); sensitivity: 28 pg/mL; (MinDC+2SD; Amylin (Active)); sensitivity: 64 pg/mL; (MinDC+2SD; Leptin); sensitivity: 7.3 pg/mL; (MinDC+2SD; GLP-1 (Total)); standard curve range: 0.69-500 pg/mL; (TNFα); standard curve range: 1.4-1,000 pg/mL; (GIP); standard curve range: 13.7-10,000 pg/mL; (Amylin (Active)); standard curve range: 13.7-10,000 pg/mL; (Amylin (Total)); standard curve range: 13.7-10,000 pg/mL; (Ghrelin (Active)); standard curve range: 13.7-10,000 pg/mL; (Glucagon); standard curve range: 13.7-10,000 pg/mL; (IL-6); standard curve range: 13.7-10,000 pg/mL; (MCP-1); standard curve range: 13.7-10,000 pg/mL; (PYY); standard curve range: 137-100,000 pg/mL; (Insulin); standard curve range: 137-100,000 pg/mL; (Leptin); standard curve range: 2.7-2,000 pg/mL; (GLP-1 (Active)); standard curve range: 2.7-2,000 pg/mL; (GLP-1 (Total)); standard curve range: 2.7-2,000 pg/mL; (PP); standard curve range: 27.4-20,000 pg/mL; (C-Peptide); inter-assay cv: <15%; intra-assay cv: <10%; (C-Peptide); inter-assay cv: <15%; intra-assay cv: <10%; (GIP); inter-assay cv: <15%; intra-assay cv: <10%; (GLP-1 (Active)); inter-assay cv: <15%; intra-assay cv: <10%; (GLP-1 (Total)); inter-assay cv: <15%; intra-assay cv: <10%; (Ghrelin (Active)); inter-assay cv: <15%; intra-assay cv: <10%; (Glucagon); inter-assay cv: <15%; intra-assay cv: <10%; (IL-6); inter-assay cv: <15%; intra-assay cv: <10%; (Insulin); inter-assay cv: <15%; intra-assay cv: <10%; (Leptin); inter-assay cv: <15%; intra-assay cv: <10%; (MCP-1); inter-assay cv: <15%; intra-assay cv: <10%; (PP); inter-assay cv: <15%; intra-assay cv: <10%; (PYY); inter-assay cv: <15%; intra-assay cv: <10%; (TNFα); inter-assay cv: <20%; intra-assay cv: <10%; (Amylin (Active)); inter-assay cv: <20%; intra-assay cv: <10%; (Amylin (Total))
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Metabolic syndrome is a cluster of conditions, including increased blood pressure, elevated insulin levels, excess body fat around the waist, and abnormal cholesterol levels. Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia, and central obesity—all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death. Adults with metabolic syndrome present a low-grade inflammation, whose link with obesity may be a production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes, and obesity related hormones, as well as other cardiovascular disease biomarkers.

The MILLIPLEX^® Human Metabolic Hormone Panel is a 15 analyte, 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Amylin (Active) or Amylin (Total), C-Peptide, Ghrelin (Active), GIP, GLP-1 (Active) or GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

• Analytes: Amylin (Active), Amylin (Total), C-Peptide, Ghrelin (Active), GIP (Total), GLP-1 (Active), GLP-1 (Total), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total), TNF-α
• NOTE: The following analytes cannot be assayed simultaneously: Amylin (Active) & Amylin (Total); GLP-1 (Active) & GLP-1 (Total).
• Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
• Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; cell/tissue culture samples may be used undiluted or diluted with an appropriate control medium NOTE: The following analytes require the addition of protease inhibitors to serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor).
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Metabolic Disorders

Download the HMHEMAG-34K xPonent Standard Template Version Magpix

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Linkage

Replaces: HMHMAG-34K

Other Notes

Please contact Technical Service for linearity of dilution.

Please note: Amylin (Active) and Amylin (Total) cannot be plexed together. GLP-1 (Active) and GLP-1 (Total) cannot be plexed together.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H302 + H332,H311,H317,H319,H411
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects