HCVD3MAG-67K

MILLIPLEX^® Human Cardiovascular Disease (Acute Phase) Magnetic Bead Panel 3 - Cardiovascular Disease Multiplex Assay

The analytes available for this multiplex kit are: Adipsin, AGP, α2-Macroglobulin, CRP, Fetuin A, Fibrinogen, L-Selectin, Serum Amyloid P, Haptoglobin, & Platelet Factor-4.

• Synonym(s): human CVD APP immunoassay panel, luminex human cardiovascular disease acute phase proteins multiplex assay, millipore human CVD acute phase proteins multiplex kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 102%; (Haptoglobin); accuracy: 109%; (AGP); accuracy: 113%; (Platelet Factor-4); accuracy: 117%; (Adipsin); standard curve range: 0.004-15 ng/mL; (L-Selectin & SAP); standard curve range: 0.012-50 ng/mL; (CRP); standard curve range: 0.037-150 ng/mL; (PF4); standard curve range: 0.061-250 ng/mL; (HPTGN); standard curve range: 0.098-400 ng/mL; (AGP & Adipsin); standard curve range: 0.244-1,000 ng/mL; (Fetuin A & vWF); standard curve range: 0.488-2,000 ng/mL; (A2M)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Cardiovascular disease, particularly atherosclerotic vascular disease, is a leading cause of global mortality, accounting for 30% of all global deaths (WHO, 2010). Inflammatory mechanisms play a vital role in the initiation, maintenance, and progression of vascular disease with a strong correlation between inflammatory markers and prognosis in acute and chronic coronary artery disease. Numerous studies have demonstrated an association of obesity and diabetes with cardiovascular risk factors.

MILLIPLEX^® Human Cardiovascular Disease (CVD) Panel 3 (Acute Phase) is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma or tissue/cell lysate and culture supernatant samples: Adipsin, α-1-Acid-Glycoprotein (AGP), α-2-Macroglobulin (A2M), C Reactive Protein (CRP), Fetuin A, Fibrinogen, L-Selectin, Serum Amyloid P (SAP), Haptoglobin, and Platelet Factor-4 (PF4). (Please note: Fibrinogen is not detectable in serum.) This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular

Application

• Analytes: α-1 Acid Glycoprotein (AGP), α-2-Macroglobulin (A2M), Adipsin (Factor D), C Reactive Protein (CRP), Fetuin A, Fibrinogen, Haptoglobin, sL-Selectin, Platelet Factor-4 (PF4/CXCL4), Serum Amyloid P (SAP)
• Recommended Sample Type: Human serum, plasma, cell/tissue culture supernatants and lysates
• Recommended Sample Dilution: 25 μL per well of 1:40,000 diluted serum or plasma; tissue/cell culture samples may require dilution in appropriate control medium
• NOTE: Fibrinogen is not detectable in serum
• Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25 °C)
• Research Category: Cardiovascular Disease
• Research Subcategory: Metabolic Disorders

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual analyte sensitivity.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H302 + H332,H311,H315,H317,H319,H335,H411
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects