HBNMAG-51K

MILLIPLEX^® Human Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay

The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN - Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α.

• Synonym(s): human bone metabolic biomarkers immunoassay panel, luminex human bone metabolism multiplex assay, millipore human bone metabolic biomarkers multiplex kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 82-97%; standard curve range: 0.24-1,000 pg/mL; (TNFα); standard curve range: 1-3,000 pg/mL; (IL-1β); standard curve range: 1-6,000 pg/mL; (ACTH & IL-6); standard curve range: 146-600,000 pg/mL; (OC); standard curve range: 24-100,000 pg/mL; (SOST); standard curve range: 37-150,000 pg/mL; (FGF-23); standard curve range: 49-200,000 pg/mL; (Leptin); standard curve range: 5-20,000 pg/mL; (DKK-1); standard curve range: 5-20,000 pg/mL; (PTH); standard curve range: 61-250,000 pg/mL; (Insulin); standard curve range: 7-30,000 pg/mL; (OPG); standard curve range: 98-400,000 pg/mL; (OPN)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation. The disruption of bone metabolism results in such disease as osteoporosis, osteoarthritis, rheumatoid arthritis, chronic kidney disease and bone metastases.

MILLIPLEX^® Human Bone Magnetic Bead kit is to be used for the simultaneous quantification of any or all of the following analytics in human plasma, serum or tissue/cell lysate and culture supernatant samples: Adrenocorticotropic Hormone (ACTH), Dickkopf-1 (DKK1), IL-6, Insulin, Leptin, TNFα, Osteopotegrin (OPG), Osteocalcin (OC), Osteopontin (OPN), Sclerostin (SOST), IL-1β, Parathyroid Hormone (PTH) and FGF-23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Bone

Specificity

Cross Reactivty
No cross reactivity between the antibodies for an analyte in this panel were observed.

Application

• Analytes: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin (OC), Osteopontin (OPN), Osteoprotegerin (OPG), PTH, Sclerostin (SOST), TNFα
• Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or extracts
• Recommended Sample Dilution: 25 μL per well of d1:2 diluted serum or plasma; cell/tissue culture samples may be used neat or diluted in appropriate control medium
• Assay Run Time: Overnight (16–18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
• Research Category: Metabolism
• Research Subcategory: Bone

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Linkage

Replaces: HBN1B-51K

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual analytes sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H302 + H332,H311,H317,H318,H373,H411
• pcodes: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• target_organs: Respiratory Tract
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects